human normal mammary gland epithelial cell line mcf 10a  (ATCC)

Journal: SLAS technology

Article Title: CircTNFRSF19 facilitates triple negative breast cancer cell growth by regulating N6-methyladenosine modification of B3GNT5: Medical biological image simulation.

doi: 10.1016/j.slast.2025.100308

Figure Lengend Snippet: Fig. 1. CircTNFRSF19 is highly expressed in TNBC tissues and cells. (A) CircRNA expression in TNBC tissues and normal breast tissues was displayed as volcano plot based on GEO datdbase (ID: GSE101124). (B) RT-qPCR was conducted to measure the expression of circTNFRSF19 in human normal mammary epithelial cell line (MCF-10A) and human TNBC cell lines (MDA-MB-231, MDA- MB-468, BT-549 and SUM-159). (C) PCR-AGE assay was carried out to validate the loop structure of circTNFRSF19 in MDA-MB-231 and BT-549 cells. (D) The expression of circTNFRSF19 and TNFRSF19 mRNA was examined via RT-qPCR in TNBC cells with or without RNase R treatment. The experiments were carried out in triplicate. **P < 0.01.

Article Snippet: The human normal mammary gland epithelial cell line, MCF-10A, along with several established human triple-negative breast cancer (TNBC) cell lines, specifically MDA-MB-231, MDA-MB-468, BT-549, and SUM-159, were all obtained from the American Type Culture Collection (ATCC), located in Manassas, Virginia, USA.

Techniques: Expressing, Quantitative RT-PCR

Journal: Nature immunology

Article Title: Oncogenic LncRNA downregulates cancer cell antigen presentation and intrinsic tumor suppression

doi: 10.1038/s41590-019-0400-7

Figure Lengend Snippet: a , Top panel: graphic illustration of tissue-specific expression of human LINK-A gene. Bottom panel: DNA agarose gel indication of Tg( LINK-A ) genotyping. Three independent experiments were performed and yielded similar results. b , Northern blot detection of LINK-A or Actin using in vitro transcribed LINK-A sense, antisense or total RNA extracted from Tg( LINK-A ) or MMTV-Tg( LINK-A ) mammary gland. Three independent experiments were performed and yielded similar results. c , Representative images of Tg( LINK-A ) or tumor-bearing MMTV-Tg( LINK-A ) mice. Blue arrows: mammary gland tumors. Three independent experiments were performed and yielded similar results. d and e , H&E staining (d) or statistical analysis (e) of MMTV-Tg( LINK-A ) mammary gland of animals at 2, 3, 4, or 5 month of age to indicate the development from normal-like breast epithelial cells through hyperplasia, atypical hyperplasia, DCIS and IDCs. Scale bar: 100 μm (d). Error bars (e): S.E.M. n=9, 15, 15, 11 animals respectively, unpaired Student’s t-test. f-h , Kaplan-Meier survival analysis of MMTV-cre, MMTV-Tg( LINK-A ) or Tg( LINK-A ) mice according to the presence of hyperplasia (f), DCIS (g) or IDC (h). n=16, 33, 17 (f) 19, 46, 23 (g) or 18, 79, 16 (h) animals respectively (log rank test). i , Representative images of MMTV-Tg( LINK-A ) primary tumor and lung metastasis. T: tumor; N: adjacent normal tissue. Three independent experiments were performed and yielded similar results. Scale bar: 100 μm. j-l , Representative images of Tg( LINK-A ) or MMTV-Tg( LINK-A ) lung (j). Three independent experiments were performed and yielded similar results. Lung metastasis incidence of tumor-bearing mice (k) or metastatic nodules/section (l) of MMTV-cre, Tg( LINK-A ) or MMTV-Tg( LINK-A ) lung (n=18, 16, 53). Results are mean ± s.d.. P values were determined by unpaired two-tailed Student’s t test. Scale bar: 1mm (j).

Article Snippet: Human TNBC cell lines: MDA-MB-231, MDA-MB-468, BT549, HCC-1187; human HER2-positive breast cancer cell lines: BT474, SK-BR-3; human ER-positive breast cancer cell lines: MCF7, T47D, ZR-75–1; human normal mammary gland epithelial cell line: MCF10A and mouse mammary gland epithelial cell line: NMuMG were purchased from American Type Culture Collection (ATCC); SUM-149 (human TNBC cell line), B16-OVA (mouse melanoma cell line, a gift from H. Patrick), and B16F10 (mouse melanoma cell line) were maintained using standard media and conditions and the Characterized Cell Line Core Facility (MD Anderson Cancer Center).

Techniques: Expressing, Agarose Gel Electrophoresis, Northern Blot, In Vitro, Staining, Two Tailed Test